Assistant Professor of Surgery
Assistant Professor of Surgery Director, Islet Transplantation
5841 S. Maryland Avenue, Rm J517, MC 5026
Chicago, IL 60637
Phone: 773-702-6104
Email:
Education
M.D.: Duke University, Durham, NC
Residency: University of California, San Diego, CA
Fellowship: University of California, San Diego, CA Transplantation
Board Certification: Surgery
Fellowship: Duke University, Durham, NC Islet research
• Clinical
Interests
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Bibliography
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We
are a team of scientists working in collaboration with transplant clinical
staff to provide human islet cells from donated
organs to subjects enrolled in our clinical trial. Our goal is to
relieve the dangerous side effects of diabetes type I by providing these
patients with a supply of endogenous insulin via transplantation.
Our primary interest is to constantly improve the islet isolation process by testing and innovation in the laboratory. We then translate our findings to our cGMP islet isolation facility. Here we collaborate with the GMP (Good Manufacturing Process) team that assures all necessary quality controls. Our team isolates day and night during prefixed call schedules.
Both
our GMP facility and basic science laboratory are located in the lower
level of the Kovler building (910 East 58th street, Chicago IL 60637).
On the left is a picture of the Kolver Research Building.
On
the right you can see inside our GMP facility, where five main processes
are performed. First the organ is received, cleaned and separated from
spleen and duodenum. Second, a collagenase
enzyme is infused under pressure into the pancreatic duct. Third, the
`pancreas is digested into a “cellular soup”. Fourth, pancreatic
islets are purified from the acinar
tissue via a density gradient. Finally, the islets are placed in cell
incubators until they are ready for use.
We
are focusing our research on islet encapsulation using various materials
and methods. On the right is a micrograph of a human islet (indicated
by the black arrow) still within the pancreas tissue. The next micrograph
below shows an isolated rodent islet before encapsulation.
Similar
techniques are applied for the isolation of rodent and human islets. The
encapsulation process may provide an alternative to the administration
of immune suppressive drugs in transplanted patients. Encapsulation acts
as a barrier against the transplanted host immune system.
At
the same time this allows nutrients and waste to be freely exchanged.
On the right, the micrograph shows rodent islets encapsulated via the
selective withdrawal method.
Another
encapsulation material is shown to the left. The capsules are much larger,
can contain many islets and are made from an algae derivative. In the
laboratory we evaluate the viability and insulin release of these preparations
in vitro. We then proceed to in vivo implantation studies.
We
also look at integration and evaluate fibrosis generated by the various
materials. An example is shown on the micrograph to the right. The black
arrow indicates cellular fibrosis around an algae derived capsule. Other
subjects of interest are transplantation site of injection and collaboration
on human islet physiology at the University of Chicago Medical Center.
TEAM 2007
From the left to the right, N.Younes M.D. (visiting professor from Jordan and Fulbright Scholar), S.Liu M.D. Ph.D. (Team Senior), D. Ostrega, M.B.A (cGMP Director), J-M Nothias M.S. (Team Chief), M. Shah B.A. (Team Junior).
TEAM 2008
From the left to the right, M. Umehara M.D. (visiting surgeon from Japan), S.Liu M.D. Ph.D. (Team Senior), D. Ostrega, M.B.A (cGMP Director), J-M Nothias M.S. (Team Chief), A. Scavone M.S.E. (Team Junior).
